Antibody Engineering A Practical Approach
by McCafferty, John; Hoogenboom, Hennie R.; Chiswell, David J.Format: Hardcover
Pub. Date: 1996-08-29
Publisher(s): Oxford University Press
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Summary
Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years. This has resulted in rapid changes in how to handle antibodies for research. This new Practical Approach volume responds to this change by assembling, in one volume, protocols which allowthe researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities. The book is divided into two parts: the first describes the generation and analysis of antibodies and the second covers engineering and production.This timely book will be of interest to those involved in both clinical and molecular biological applications of antibodies.
Table of Contents
| Construction and use of antibody gene repertoires | |
| Affinity maturation of antibodies using phage display | |
| Human antibody repertoires in transgenic mice: manupulation and transfer of YACs | |
| Measuring antibody affinities in solution | |
| Measuring antibody affinities using Bio-sensors | |
| Analysis of human antibody sequences | |
| Rodent to human antibodies by CDR grafting | |
| Rodent to human antibodies by phage display | |
| Choosing and manipulating effector functions | |
| Secretion and purification of antibody fragments expressed in bacteria | |
| Production of single chain Fv monomers and multimers | |
| Expression of immunoglobulin genes in mammalian cells | |
| In vitro linkage of antibody fragments into dimeric and multimeric forms | |
| Table of Contents provided by Publisher. All Rights Reserved. |
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