Current Protocols Essential Laboratory Techniques
by Gallagher, Sean R.; Wiley, Emily A.Edition: 1st
Format: Paperback
Pub. Date: 2008-02-04
Publisher(s): Current Protocols
Other versions by this Author
List Price: $112.00
Buy New
Usually Ships in 3-4 Business Days
$111.89
Rent Textbook
Select for Price
Used Textbook
We're Sorry
Sold Out
eTextbook
We're Sorry
Not Available
How Marketplace Works:
- This item is offered by an independent seller and not shipped from our warehouse
- Item details like edition and cover design may differ from our description; see seller's comments before ordering.
- Sellers much confirm and ship within two business days; otherwise, the order will be cancelled and refunded.
- Marketplace purchases cannot be returned to eCampus.com. Contact the seller directly for inquiries; if no response within two days, contact customer service.
- Additional shipping costs apply to Marketplace purchases. Review shipping costs at checkout.
Summary
2008 PROSE award winner for Excellence in Biology and the Life SciencesFrom the leading branded source for methods in the life sciences, this essential resource for the lab provides every researcher with the skills and understanding of fundamental laboratory procedures to ensure greater success at the bench. It takes the novice researcher from the very basics of reagent preparation to the use of routine instrumentation found in most life science research laboratories around the world. Developed by Current Protocols, the most trusted and recognized source of protocols Teaches new investigators how to perform basic laboratory research techniques Describes solution chemistry and preparation Covers basic laboratory safety Describes care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes Teaches how to manage information from lab notebooks, images, literature references, as well as manuscript preparationThis book is an invaluable resource for researchers in all areas of the life sciences, especially molecular biology, biotechnology, genetics, and immunology. It is essential reading for undergraduate and graduate students alike and is also of interest for investigators new to life science research.
Author Biography
Sean R. Gallagher, Ph.D. is the Vice President and Chief Technology Officer for UVP, LLC (Upland, CA), where he oversees applied technology and product development for specialty light source and life science imaging products. He is on the corporate advisory councils of the Keck Graduate Institute of Applied Life Sciences and Harvey Mudd College (Claremont, CA) and has eight patents on technologies he has codeveloped. He received his doctorate in Botany from the University of California at Riverside and completed a post-doctoral position at Stanford University.
Emily A. Wiley, Ph.D. is an Assistant Professor in the Joint Science Department of Claremont McKenna, Pitzer, and Scripps Colleges (Claremont, CA), where she is actively involved in teaching undergraduate laboratory-intensive courses and mentoring students in original research. She received her doctorate in the area of molecular genetics from the University of Washington and held postdoctoral research positions at the University of Rochester and at The Fred Hutchinson Cancer Research Center in Seattle She maintains an active research program studying chromatin assembly and modification in Tetrahymena thermophila, which serves the dual function of providing undergraduates the opportunity to engage in modern life science.
Table of Contents
Foreword.
Preface.
Contributors.
Common Conversion Factors.
Combining Techniques to Answer Molecular Questions.
Chapter 1: Volume/Weight Measurement.
1.1 Volume Measurement.
1.2 Weight Measurement.
Chapter 2: Concentration Measurement.
2.1 Spectrophotometry.
2.2 Quantitation of Nucleic Acids and Proteins.
2.3 Radiation Safety and Measurement.
Chapter 3: Reagent Preparation.
3.1 Reagent Preparation: Theoretical and Practical Discussions.
3.2 Measurement of pH.
3.3 Recipes for Commonly Encountered Reagents.
Chapter 4: Cell Culture Techniques.
4.1 Aseptic Technique.
4.2 Culture of Escherichia coli and Related Bacteria.
Chapter 5: Sample Preparation.
5.1 Centrifugation.
5.2 Purification and Concentration of Nucleic Acids.
Chapter 6: Chromatography.
6.1 Overview of Chromatography.
6.2 Column Chromatography.
Chapter 7: Electrophoresis.
7.1 Overview of Electrophoresis.
7.2 Agarose Gel Electrophoresis.
7.3 SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
7.4 Staining Proteins in Gels.
7.5 Overview of Digital Electrophoresis Analysis.
Chapter 8: Blotting.
8.1 Overview of Blotting.
8.2 Nucleic Acid Blotting: Southern and Northern.
8.3 Protein Blotting: Immunoblotting.
8.4 Labeling DNA and Preparing Probes.
Chapter 9: Microscopy.
9.1 Conventional Light Microscopy.
9.2 Immunofluorescence Microscopy.
Chapter 10: Enzymatic Reactions.
10.1 Working with Enzymes.
10.2 Overview of PCR.
10.3 Real-Time PCR.
10.4 DNA Sequencing: An Outsourcing Guide.
Appendices.
A.1 Laboratory Safety.
A.2 Laboratory Notebooks and Data Storage.
A.3A Ethical Considerations When Altering Digital Images.
A.3B Practical Considerations When Altering Digital Images.
A.4 Statistical Analysis.
A.5A Preparing and Presenting a Poster.
A.5B Preparing and Presenting a Talk.
Index.
An electronic version of this book is available through VitalSource.
This book is viewable on PC, Mac, iPhone, iPad, iPod Touch, and most smartphones.
By purchasing, you will be able to view this book online, as well as download it, for the chosen number of days.
Digital License
You are licensing a digital product for a set duration. Durations are set forth in the product description, with "Lifetime" typically meaning five (5) years of online access and permanent download to a supported device. All licenses are non-transferable.
More details can be found here.
A downloadable version of this book is available through the eCampus Reader or compatible Adobe readers.
Applications are available on iOS, Android, PC, Mac, and Windows Mobile platforms.
Please view the compatibility matrix prior to purchase.