Protein Kinase C Protocols,9781588290687

Protein Kinase C Protocols

by
ISBN13: 9781588290687
ISBN10: 1588290689
Format: Hardcover
Pub. Date: 2003-06-01
Publisher(s): Humana Pr Inc
List Price: $199.00

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Summary

The first compilation of classic and emerging laboratory techniques for the study of the biochemistry and cell biology of protein kinase C (PKC). Described in step-by-step detail, these methods can be easily used to explore the structure, function, regulation, subcellular localization, and macromolecular interactions of protein kinase C.. Each protocol is introduced in the context of PKC function and regulation and contains many notes on how best to deal with the problems that may occur. Comprehensive and authoritative, Protein Kinase C Protocols is a timely compilation of biophysical, biochemical, cell biological, and molecular biological approaches that brings protein kinase C research into any laboratory interested in studying it.

Table of Contents

Preface vii
Contributors xiii
PART I. INTRODUCTION
1 The Ins and Outs of Protein Kinase C
Alexandra C. Newton
3(6)
2 Early Studies of Protein Kinase C: A Historical Perspective
Yasutomi Nishizuka and Ushio Kikkawa
9(12)
PART II. EXPRESSION AND PURIFICATION
3 Expression and Purification of Protein Kinase C from Insect Cells
Hideyuki Mukai and Yoshitaka Ono
21(14)
4 Expression and Purification of Protein Kinase Cð from Bacteria
Michael Gschwendt and Luise Stempka
35(10)
PART III. MEASURING THE ACTIVITY OF PROTEIN KINASE C
5 Complexities in Protein Kinase C Activity Assays: An Introduction
Julianne J. Sando
45(18)
6 Enzyme Assays for Protein Kinase C Activity
Julianne J. Sando and Jacquelyn K. Beats
63(14)
7 Subcellular Translocation of Protein Kinase C
Julianne J. Sando, Jacquelyn K. Beats, and Isa M. Hussaini
77(12)
PART IV. MEASURING THE INTERACTION OF PROTEIN KINASE C WITH MEMBRANES
8 Measuring the Interaction of Protein Kinase C with Membranes: An Introduction
Alexandra C. Newton
89(4)
9 Fluorescence Imaging of Protein Kinase C Translocation in Living Cells
Naoaki Saito
93(12)
10 Measuring the Binding of Protein Kinase C to Sucrose-Loaded Vesicles
Jennifer Giorgione and Alexandra C. Newton
105(10)
11 Use of Stopped-Flow Fluorescence Spectroscopy to Measure Rapid Membrane Binding by Protein Kinase C
Eric A. Nalefski and Alexandra C. Newton
115(14)
12 [3H]Phorbol 12,13-Dibutyrate Binding Assay for Protein Kinase C and Related Proteins
Nancy E. Lewin and Peter M. Blumberg
129(30)
PART V. MEASURING THE PHOSPHORYLATION OF PROTEIN KINASE C
13 Protein Kinase C Phosphorylation: An Introduction
Peter J. Parker
159(4)
14 Pulse-Chase Analysis of Protein Kinase C
Mikiko Takahashi and Yoshitaka Ono
163(8)
15 PDK-1 and Protein Kinase C Phosphorylation
Alex Toker
171(20)
16 In Vitro Autophosphorylation of Protein Kinase C Isozymes
Antonio M. Pepio and Wayne S. Sossin
191(16)
17 Tyrosine Phosphorylation of Protein Kinase C
Toshiyoshi Yamamoto, Emiko Yamauchi, Hisaaki Taniguchi, Hidenori Matsuzaki, and Ushio Kikkawa
207(10)
18 Methods to Study Dephosphorylation of Protein Kinase C In Vivo
Karen England and Martin Rumsby
217(16)
19 Phosphopeptide-Specific Antibodies to Protein Kinase C
Wayne S. Sossin
233(14)
PART VI. IDENTIFYING PROTEIN KINASE C SUBSTRATES
20 Identifying Protein Kinase C Substrates: An Introduction
Alex Toker
247(6)
21 A Chemical Genetic Approach for the Identification of Direct Substrates of Protein Kinases
Kavita Shah and Kevan M. Shokat
253(20)
22 Studying the Optimal Peptide Substrate Motifs of Protein Kinase C Using Oriented Peptide Libraries
Michael B. Yaffe
273(16)
PART VII. STRUCTURAL ANALYSIS OF PROTEIN KINASE C
23 Structural Analysis of Protein Kinase C: An Introduction
James H. Hurley
289(2)
24 Bacterial Expression and Purification of C1 and C2 Domains of Protein Kinase C Isoforms
Wonhwa Cho, Michelle Digman, Bharath Ananthanarayanan, and Robert V. Stahelin
291(8)
25 Crystallization of the Protein Kinase CS CB Domain
Gongyi Zhang and James H. Hurley
299(8)
PART VIII. METHODS FOR DETECTING BINDING PROTEINS
26. Methods for Detecting Binding Proteins: An Introduction
Gerda Endemann and Dana Mochly-Rosen
307(20)
27. Detection of Protein Kinase-Binding Partners by the Yeast Two-Hybrid Analysis
Betty Y. Chang and Christine A. Cartwright
327(18)
28 Glutathione 5-Transferase Pull-Down Assay
Deborah Schechtman, Dana Mochly-Rosen, and Dorit Ron
345(6)
29 Overlay Method for Detecting Protein-Protein Interactions
Deborah Schechtman, Christopher Murriel, Rachel Bright, and Dana Mochly-Rosen
351(8)
30 An Overlay Assay for Detecting Protein Kinase C-Binding Proteins and Substrates
Susan Jaken
359(10)
31 Functional Proteomic Analysis of the Protein Kinase C Signaling System
Jason M. Pass, Jun Zhang, Thomas M. Vondriska, and Peipei Ping
369(20)
PART IX. PHARMACOLOGICAL PROBES FOR PROTEIN KINASE C
32 Pharmacological Probes for Protein Kinase C: An Introduction
Marcelo G. Kazanietz
389(8)
33 Applications of Inhibitors for Protein Kinase C and Their Isoforms
Garry X. Shen, Kerrie J. Way, Judith R. C. Jacobs, and George L. King
397(26)
34 Phorbol Esters as Probes for the Study of Protein Kinase C Function
Marcelo G. Kazanietz and Patricia S. Lorenzo
423(18)
35 Irreversible Inactivation of Protein Kinase C Isozymes by Thiol-Reactive Peptide Substrate Analogs
Catherine A. O'Brian, Nancy E. Ward, Feng Chu, and Jubilee R. Stewart
441(12)
PART X. GENETIC APPROACHES TO STUDYING PROTEIN KINASE C
36 Genetic Approaches to Studying Protein Kinase C: An Introduction
Robert O. Messing
453(2)
37 Animal Models in the Study of Protein Kinase C Isozymes
Doo-Sup Choi and Robert O. Messing
455(20)
38 Genetic Manipulation of Protein Kinase C In Vivo
Alex Toker
475(16)
39 Yeast as a Host to Screen for Modulators and Regulatory Regions of Mammalian Protein Kinase C Isoforms
Amadeo M. Parissenti and Heimo Riedel
491(28)
PART XI. PROTEIN KINASE C IN DISEASE
40 Protein Kinase C in Disease: Cancer
Alan P. Fields and W. Clay Gustafson
519(20)
41 Characterization of the Role of Protein Kinase C Isozymes in Colon Carcinogenesis Using Transgenic Mouse Models
Alan P. Fields, Nicole R. Murray, and W. Clay Gustafson
539(16)
42 Alcohol Addiction
M. Foster Olive and Dorit Ron
555(16)
Index 571

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