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| Preface | |
| Acknowledgements | |
| The behaviour of proteins at interfaces in relation to their structural stability | p. 3 |
| Infuence of the solvent properties on protein stability in organic media | p. 13 |
| Interfacial damage to proteins during intensive mixing in fermentation and downstream processing | p. 21 |
| Thermal stability of enzymes: influence of solvatation medium (a Raman spectroscopy study) | p. 29 |
| Protein stability in non-aqueous media: a DSC study | p. 37 |
| Stable enzymes by water removal | p. 45 |
| Enzyme stabilization by multipoint covalent attachment to activated pre-existing supports | p. 55 |
| Crosslinked enzyme crystals (CLECs) as immobilized enzyme particles | p. 63 |
| Stabilization of proteins by chemical methods | p. 75 |
| Stability of engineered antibody fragments | p. 81 |
| Structural determinants of the thermostability of thermolysin-like Bacillus neutral proteases | p. 91 |
| Molecular aspects of proteolysis of globular proteins | p. 101 |
| Stability of industrial enzymes | p. 111 |
| Stabilization of the detergent protease Savinase by proline substitution | p. 133 |
| Lessons from industry | p. 145 |
| Stabilization of enzymes by their specific antibodies | p. 153 |
| Immobilisation of [alpha]-chymotrypsin on soluble acrylic microgels; activity and stabilization | p. 167 |
| Improved stability of lignin peroxidase by immobilization | p. 175 |
| Ca[superscript 2+]-induced enhancement of the molecular stability of Pseudomonas lipases | p. 181 |
| Denaturation of ribonucleases from different sources in the presence of denaturing or stabilizing agents | p. 189 |
| Conformational dynamics of native, compact and fully unfolded states of proteins detected by frequency domain fluorometry | p. 197 |
| Characteristics, protein engineering and applications of psychrophilic marine proteinases from Atlantic cod | p. 205 |
| Low temperature inactivation of a bacterial protease | p. 215 |
| Chemical deglycosylation of Horseradish peroxidase and surglycosylation using a new glycosylating reagent: effects on catalytic activity and stability | p. 223 |
| Engineering stability and specificity of the Lactococcus lactis SK11 proteinase | p. 231 |
| Comparative studies on the thermophilicity and stability of '5-methylthioadenosine phosphorylase from various sources | p. 239 |
| A process for stabilization of glycoproteins | p. 247 |
| Pressure-induced structural modifications of butyrylcholinesterase | p. 255 |
| Changing the thermostability of Bacillus licheniformis [alpha]-amylase | p. 261 |
| Stabilization of lipases for hydrolysis reactions on industrial scale | p. 269 |
| Cold denaturation of proteins as investigated by subzero transverse temperature gradient gel electrophoresis | p. 275 |
| Genetic algorithms as a new tool to study protein stability | p. 283 |
| Modeling three-dimensional structure and electrostatics of alkali-stable cyclomaltodextrin glucanotranferase | p. 291 |
| The effect of metal ion binding on protein stability | p. 299 |
| The number of cooperative regions (energetical domains) in a pepsin molecule depends on the pH of the medium | p. 309 |
| Stabilization of soluble proteins by intramolecular crosslinking with polyfunctional macromolecules. Poly-(glutaraldehyde-like) structure | p. 315 |
| Development of a method for the stabilization and formulation of xylanase from Trichoderma using experimental design | p. 323 |
| New technique for monitoring interfacial inactivation of enzymes by organic solvents | p. 329 |
| The stabilization of analytical enzymes using polyelectrolytes and sugar derivatives | p. 337 |
| Pressure effect on the stability of lipoxygenase: FTIR studies with the diamond anvil cell | p. 347 |
| Stability of Ca[superscript 2+]-binding mutants of human lysozyme | p. 353 |
| Storage stability of enzymes in dry apolar solvent | p. 361 |
| Pressure sensitivity of enzymes and their modification | p. 369 |
| Comparative study of thermostability and structure of close homologs - barnase and binase | p. 377 |
| Immobilized Concanavalin A decreases the stability at proteolysis of amine oxidases | p. 383 |
| Stabilization of lipase from Candida rugosa by covalent immobilisation | p. 391 |
| Isolation, characterization and immobilization of penicillin acylase from Escherichia coli B-130 | p. 399 |
| Thermodynamic properties of apocytochrome P450 [subscript cam] | p. 407 |
| Stabilization of yeast D-amino acid oxidase by matrix covalent attachment | p. 415 |
| Stability of an entrapped-cell system for the [actual symbol not reproducible] of steroids in organic medium | p. 421 |
| The kinetics of enzyme inactivation | p. 429 |
| Studies on stability of S-adenosylhomocysteine hydrolase from Sulfolobus solfataricus, a thermophilic archaebacterium | p. 437 |
| Temperature, pH and media influence of lipase stability | p. 445 |
| Prediction of the unfolding heat capacity change of proteins | p. 451 |
| Inactivation of [alpha]-amylase from Bacillus amyloliquefaciens at low moisture contents | p. 459 |
| Kinetics of high-temperature inactivation of extracellular protease from Pseudomonas fluorescens 22F | p. 467 |
| Stability of a Fusarium solani pisi recombinant cutinase in reversed micelles | p. 473 |
| Influence of long chain alcohols and chemical modification on the microencapsulated [alpha]-chymotrypsin stability | p. 481 |
| Stability of A. oryzae [beta]-galactosidase in water miscible organic solvents | p. 489 |
| Fixation of the unfolding region - a hypothesis of enzyme stabilization | p. 497 |
| Probing conformational transitions in interface [alpha]1[beta]2 of human hemoglobin by site-directed mutagenesis | p. 505 |
| Chemical modification of protein molecules improve their activity in organic solvents | p. 511 |
| Author Index | p. 519 |
| Table of Contents provided by Blackwell. All Rights Reserved. |
Stability and Stabilization of Enzymes : Proceedings of an International Symposium Held in Maastrich, The Netherlands, 22-25 November, 1992
by Van Den Tweel, W. J. J.; Harder, A.; Buitelaar, R. M.Format: Hardcover
Pub. Date: 1993-05-01
Publisher(s): Elsevier Science Ltd
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